Maya selling sunset reunion meme8/23/2023 She allegedly asked the other estate agents not to like Chrishell’s Instagram posts as well. Christine pulled out her petty cards by naming one of the drinks “Chrishell’s Two-Faced Tonic”, for a party she hosted towards the end of season 1. The animosity continued after the cameras stopped rolling. The feud ended with Christine calling Chrishell a “b*tch who must own a Sunglasses Hut because she is so f*cking shady.” Ouch! Christine, who was Mary’s best friend at the time, did not take this interrogation lightly, standing up for her colleague and bestie. Questions about Romain’s financial conditions and their 10-year age gap were discussed. The first season navigated murky waters as soon as Davina and Chrishell’s conversation about Mary and Romain’s newly-engaged relationship came out in the open. Christine Vs Chrishell over Mary and Romain’s relationship A moment from Christine and Chrishelle’s showdown in season 1. In season 4, Christine and Emma were at loggerheads when both ladies realised that they were once dating the same ex.Ĭonsidering each season has brought its own degree of drama, we dissect the most shocking, controversial fights on Selling Sunset. Davina and Mary’s partner, Romain, were also involved in a heated argument, leading to Davina being uninvited from their wedding. The first season was headlined by Christine’s s hostility towards Chrishell, accusing the latter of being “two-faced” at every opportunity. Since the show successfully completed a six season run, it is no secret that plenty of arguments have been telecast. Which were the most dramatic fights on Selling Sunset? From $75 million listings to properties overlooking LA’s iconic Hollywood Hills, the opulence per square metre is too colossal to deny. While a handful of reality real-estate dramas have come and gone (Think Million Dollar Listing, Fixer Upper, The World’s Most Extraordinary Homes), what sets Selling Sunset apart is its luxurious mix of heel-tapping, Chanel-wearing realtors with a showcase of California’s most decadent homes. Why do you need to watch all seasons of Selling Sunset? The cast of season 6 of Selling Sunset. Where’s the fun of unscripted primetime shows if not peppered with scandalous, jaw-dropping moments? Interestingly, shows that have gone on to garner pop-culture clout have all witnessed their fair share of controversial feuds, fights and drama, Selling Sunset included.įrom Christine Quinn hurling accusations against Season 1 newbie Chrishelle Strauss to Davina Potratz’s fall-out with Jason Oppenheim, the fights on Selling Sunset have kept reality television enthusiasts on the edge of their seats. Reality television and controversy have always gone hand-in-hand.
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Notable achievements include the ability to investigate local fitness landscapes of proteins or nucleic acids, to perform in-depth structure to function studies, and to analyse local and global regions of proteins 12, 13, 14, 15.Īt the forefront of NGS technologies are Illumina sequencing methods, which can sequence 150–600 base pairs (bp) and up to one billion sequences 16. However, NGS technologies have enabled simultaneous high-throughput sequencing of millions to billions of DNA molecules and allowed for a more comprehensive analysis of selection and screening outputs, thereby probing a greater proportion of the potential sequence space 10, 11. Such studies face challenges resulting from the limitations of DNA-sequencing technologies, in particular when reliant on laborious and expensive Sanger sequencing-based methods 8, 9. In molecular engineering, large mutational libraries of DNA encoding protein variants are frequently used to select, screen, or probe for specific properties or activities 6, 7. Consequently, advances in DNA sequencing technology bring benefits to many fields of research, including molecular engineering 5. Next generation sequencing (NGS) approaches have improved our ability to study genomes and large libraries of DNA for a fraction of previous costs 2, 3, 4. Over the past decade, advances in DNA sequencing technology have accelerated at an unprecedented rate 1. The improved method is accompanied by a simple and user-friendly analysis pipeline, DeCatCounter, to sequence medium-length sequences efficiently at one-fifth of the cost. We applied this efficient concatenation protocol to sequence nine DNA populations from a protein engineering study. Our method improved upon previously published concatenation attempts, leading to a greater sequencing depth, high-quality reads and limited sample preparation at little expense. We optimised a simple, robust method to concatenate genes of ~ 870 bp five times and then sequenced the resulting DNA of ~ 5,000 bp by PacBioSMRT long-read sequencing. We sought to sequence several DNA populations of ~ 870 bp in length with a sequencing accuracy of 99% and to the greatest depth possible. For example, the PacBio Sequel I system yields ~ 100,000–300,000 reads with an accuracy per base pair of 90–99%. Methods for sequencing mid-sized sequences of 600–5,000 bp are currently less efficient. Main developments have focused on either sequencing many short sequences or fewer large sequences. Advances in sequencing technology have allowed researchers to sequence DNA with greater ease and at decreasing costs. 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In one study, healthy men drank a 2/3 cup (160 ml) of apple juice, then scientists drew their blood. Both of these processes are underlying factors in chronic conditions, including certain cancers and heart disease ( 8). These plant compounds may protect your cells from inflammation and oxidative damage. While the majority of these compounds are in the peel, some from the apple flesh are retained in the juice ( 7). To avoid sideĮffects, dilute to half-strength when using it to rehydrate after an illness.Īpples are rich in plant compounds, particularly polyphenols. Water and tastes good, making it a good choice for hydrating. Though the amount of potassium in apple juice is similar to electrolyte drinks, it has little sodium, which is also lost via bodily fluids when you’re sick ( 1, 2, 3). In more severe cases of dehydration, medicinal electrolyte drinks are still advised. They’re also relatively expensive.ĭiluted apple juice is a practical and pleasant alternative for kids, as well as adults ( 4).īe sure to drink diluted juice to rehydrate, as the high sugar content of full-strength juice can draw excess water into your gut and worsen diarrhea - particularly during recovery from illness ( 5, 6). Though electrolyte drinks are specially formulated to rehydrate, some children don’t like the taste and won’t drink them. In a study of mildly dehydrated children with diarrhea and vomiting, those offered diluted apple juice were 6.5% less likely to need fluids delivered via their veins than those given a medicinal electrolyte drink ( 4). In fact, some pediatricians recommend half-strength apple juice - a mix of half juice, half water - for sick kids who are mildly dehydrated and at least one year old ( 2, 3). This makes it easy to consume - especially for those who are sick and at an increased risk of dehydration ( 1). Apple juice is 88% water and tastes good. 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Drag your image files to the online converter, support JPG, PNG, BMP, TIF and multiple files. Besides TIFF, this tool supports JPG, BMP, GIF, and PNG. The format has evolved to allow for editing and interactive elements like electronic signatures or buttons. This free online TIFF to PDF converter allows combining multiple images into a single PDF document. It was developed by Adobe so people could share documents regardless of which device, operating system, or software they were using, while preserving the content and formatting. PDF stands for ‘Portable Document Format’ file. This compression can affect quality, but this loss of quality isn’t noticeable to most users. JPG is a ‘lossy’ format, meaning that it is compressed to save storage space and make it easier to share. to the rescue In seconds, your PDF is converted back to JPGs. JPEG stands for Joint Photographic Experts Group, which is the name of the committee that standardised the format. However, you can usually adjust settings to save into other formats if needed. Many cameras, smartphones, and basic photo or drawing programs will automatically save into JPG format. JPGs are 2D pixel-based ‘raster’ images, which makes them better for photographs or scans, rather than digital illustrations which are often better as ‘vector’ images. Wait for the conversion to finish and download the converted PDF instantly. Click 'Start Conversion' to start the online conversion. Thumbnails will show up after uploading, drag the thumbnails to adjust the order, set up the PDF page size or margin value. JPG is a universal format which can be opened by almost all image-viewing or -editing programs, by web browsers, and by certain other apps, and they’re supported by most devices. Drag your image files to the online converter, support JPG, PNG, BMP, TIF and multiple files. JPG, or JPEG, is one of the most widely used digital image formats. 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